I would like to extract AFD from a particular set of tracts that I have a priori hypotheses about and relate their AFD to behavioural measures. The documentation on the website speaks specifically to the fixel-based analysis and I was wondering if my approach is valid.
I skull stripped the diffusion weighted data and preprocessed the data with fsl topup and eddy. I ran dwi2response on all subjects in the group and then took the group average of the output. I used those group averages to calculate the FOD files for each subject with dwi2fod, Performed joint bias field correction and intensity normalisation and generated whole brain tracts and then pruned them to my desired ROIs, which I would now like to use as masks to extract AFD for each subject and relate those values to behaviour.
Will these values be relatable across subjects or does the analysis absolutely have to take place on a fixel level in template space?
3 shells, 60 directions, multiple b0’s; let me know if any other pertinent info is needed.