iFOD2 might be failed to track the simulation fiber bundle

Glad you got to the bottom of it. Indeed, the cutoff is going to be critical here. The default is set assuming your fODF has been estimated using (MSMT-)CSD with an appropriately calibrated response function, and/or intensity normalised using mtnormalise. This being simulated data, there’s a good chance the scaling differs from what’s expected, which would require a different cutoff.

Otherwise, if you used regular CSD (not the MSMT variant), the default cutoff is probably now too low for noisy data, especially if unconstrained using ACT – similar issue to this thread.