# ACT and no reversed phase-encoding information

**URL:** https://community.mrtrix.org/t/act-and-no-reversed-phase-encoding-information/1122
**Category:** Uncategorized
**Created:** [August 21, 2017, 4:20am UTC](https://community.mrtrix.org/t/act-and-no-reversed-phase-encoding-information/1122 "2017-08-21T04:20:12Z")
**Posts on this page:** 1
**Showing post:** 6

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### Author: ![ThijsDhollander](https://community.mrtrix.org/user_avatar/community.mrtrix.org/thijsdhollander/32/1617_2.png) [@ThijsDhollander](https://community.mrtrix.org/u/ThijsDhollander)
#### Post date: [October 28, 2017, 7:45am UTC](https://community.mrtrix.org/t/act-and-no-reversed-phase-encoding-information/1122/6 "2017-10-28T07:45:32Z")

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> [@mccolgan](#):
>
> Is this okay to use for single shell data as well?

Yep, no worries. But MSMT-CSD will be limited to 2 tissue types. To get 3 tissue types from such data, you’d need SS3T-CSD, which isn’t available publicly yet.

> [@mccolgan](#):
>
> I am also using mouse data for another experiment, is this okay for mouse dwi images?

Sure, no worries again, but you may want to use the `-fa` option to `dwi2response dhollander`, and provide it with a slightly lower value than the default of 0.2. See this post, where success was shown on rat data: [Multi-tissue CSD](http://community.mrtrix.org/t/multi-tissue-csd/1268) . The general advise for a mouse would be similar, but you’d probably have to be extra-careful about setting that parameter decently. Set it to a couple of values, and look at the `-voxels` output to see what makes sense (or doesn’t).

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