# Can the voxel size of the 5ttseg.mif file generated from the T1 structural image（1mm） be different from that of the align.mif（2mm） diffusion image file?

**URL:** <https://community.mrtrix.org/t/can-the-voxel-size-of-the-5ttseg-mif-file-generated-from-the-t1-structural-image-1mm-be-different-from-that-of-the-align-mif-2mm-diffusion-image-file/8639>\
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**Created:** [December 17, 2025, 3:26am UTC](https://community.mrtrix.org/t/can-the-voxel-size-of-the-5ttseg-mif-file-generated-from-the-t1-structural-image-1mm-be-different-from-that-of-the-align-mif-2mm-diffusion-image-file/8639 "2025-12-17T03:26:28Z")\
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**Author:** ![qianjinbo-creat](https://community.mrtrix.org/user_avatar/community.mrtrix.org/qianjinbo-creat/32/5312_2.png) [@qianjinbo-creat](https://community.mrtrix.org/u/qianjinbo-creat)\
**Post date:** [December 17, 2025, 3:26am UTC](https://community.mrtrix.org/t/can-the-voxel-size-of-the-5ttseg-mif-file-generated-from-the-t1-structural-image-1mm-be-different-from-that-of-the-align-mif-2mm-diffusion-image-file/8639/1 "2025-12-17T03:26:28Z")

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Can the voxel size of the 5ttseg.mif file generated from the T1 structural image（1mm） be different from that of the align.mif（2mm） diffusion image file?

code：

dwi2response msmt\_5tt align.mif 5ttseg.mif ms\_5tt\_wm.txt ms\_5tt\_gm.txt ms\_5tt\_csf.txt -voxels ms\_5tt\_voxels.mif

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**Author:** ![rpampati](https://community.mrtrix.org/user_avatar/community.mrtrix.org/rpampati/32/5335_2.png) [@rpampati](https://community.mrtrix.org/u/rpampati)\
**Post date:** [December 22, 2025, 10:38pm UTC](https://community.mrtrix.org/t/can-the-voxel-size-of-the-5ttseg-mif-file-generated-from-the-t1-structural-image-1mm-be-different-from-that-of-the-align-mif-2mm-diffusion-image-file/8639/2 "2025-12-22T22:38:28Z")

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Hey Qianjinbo,

I’m not quite sure what you mean (maybe I’m just interpreting your question wrong). The 5tt segmentation happens in T1 space and accordingly will have the same voxel size as your T1. I assume your T1 is a different resolution than your DWI, so the segmentation and DWI will have different voxel sizes.

Best,

Rishi