# Is MRtrix3 suitable for fiber-tracking analysis of MGH HCP Adult Diffusion data?
**URL:** https://community.mrtrix.org/t/is-mrtrix3-suitable-for-fiber-tracking-analysis-of-mgh-hcp-adult-diffusion-data/3647
**Category:** Uncategorized
**Created:** [May 8, 2020, 11:48am UTC](https://community.mrtrix.org/t/is-mrtrix3-suitable-for-fiber-tracking-analysis-of-mgh-hcp-adult-diffusion-data/3647 "2020-05-08T11:48:29Z")
**Posts on this page:** 3
**Page:** 1
### Author:  [@ZhangHui](https://community.mrtrix.org/u/ZhangHui)
#### Post date: [May 8, 2020, 11:48am UTC](https://community.mrtrix.org/t/is-mrtrix3-suitable-for-fiber-tracking-analysis-of-mgh-hcp-adult-diffusion-data/3647/1 "2020-05-08T11:48:29Z")
Dear experts,
I am using MGH HCP Adult Diffusion data to explore connection between the cerebellum and cerebrum. The b-values are 1000 ,3000 ,5000 and 10000 s/mm2. However, I had noted that " Constrained Spherical Deconvolution … relies on single-shell high angular resolution diffusion imaging (HARDI) data, containing at least one non-zero b-value. Ideally, the b-value used should be in the region of 2,500 – 3,000 s/mm² (at least for in vivo human brains), although good results have sometimes been obtained using b = 1000 s/mm² data."
Can MRtrix3 be used in this case? Is there any parameter needed to be adjusted?
Thank you,
Hui Zhang
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### Author:  [@bjeurissen](https://community.mrtrix.org/u/bjeurissen)
#### Post date: [May 8, 2020, 6:15pm UTC](https://community.mrtrix.org/t/is-mrtrix3-suitable-for-fiber-tracking-analysis-of-mgh-hcp-adult-diffusion-data/3647/2 "2020-05-08T18:15:01Z")
It can definitely be used! Higher b-values actually provide better angular contrast, which is what CSD relies on to separate different fiber orientations.
As the data you plan to use are multi-shell data, the best place to start is here: [https://mrtrix.readthedocs.io/en/latest/constrained\_spherical\_deconvolution/multi\_shell\_multi\_tissue\_csd.html](https://mrtrix.readthedocs.io/en/latest/constrained_spherical_deconvolution/multi_shell_multi_tissue_csd.html)
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### Author:  [@ZhangHui](https://community.mrtrix.org/u/ZhangHui)
#### Post date: [May 9, 2020, 1:12am UTC](https://community.mrtrix.org/t/is-mrtrix3-suitable-for-fiber-tracking-analysis-of-mgh-hcp-adult-diffusion-data/3647/3 "2020-05-09T01:12:35Z")
Hi, Bjeurissen,
Thanks a lot. By the way, when used for analysis of 7T dMRI from HCP, is there anything special needed to be adopted?
Best,
Hui