# Issue with mtnormalise: Non-positive tissue balance factor error

**URL:** <https://community.mrtrix.org/t/issue-with-mtnormalise-non-positive-tissue-balance-factor-error/7648>\
**Category:** Uncategorized\
**Created:** [May 20, 2024, 8:38pm UTC](https://community.mrtrix.org/t/issue-with-mtnormalise-non-positive-tissue-balance-factor-error/7648 "2024-05-20T20:38:51Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![Nagore\_Castro](https://community.mrtrix.org/user_avatar/community.mrtrix.org/nagore_castro/32/4790_2.png) [@Nagore\_Castro](https://community.mrtrix.org/u/Nagore_Castro)\
**Post date:** [May 20, 2024, 8:38pm UTC](https://community.mrtrix.org/t/issue-with-mtnormalise-non-positive-tissue-balance-factor-error/7648/1 "2024-05-20T20:38:52Z")

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Hello MRtrix3 community,

I’m encountering an error while using the `mtnormalise` command in my diffusion MRI analysis pipeline. When running the command with the following parameters:

mtnormalise wmfod.mif wmfod\_norm.mif gmfod.mif gmfod\_norm.mif csffod.mif csffod\_norm.mif -mask mask.mif

I receive the following error message:

mtnormalise: [ERROR] Non-positive tissue balance factor was computed. Balance factors: 4.9786114020087711 0 0.0055108507153780427

It seems that one of the tissue balance factors is zero or negative, causing the error. I’ve checked my input data, ensured the mask is correctly defined, and reviewed the parameters used with `mtnormalise`, but I’m still unable to resolve the issue.

Could someone please provide guidance on how to troubleshoot and resolve this error? Any insights or suggestions would be greatly appreciated.

Thank you in advance for your help!

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**Author:** ![polivaoren](https://community.mrtrix.org/letter_avatar_proxy/v4/letter/p/e47774/32.png) [@polivaoren](https://community.mrtrix.org/u/polivaoren)\
**Post date:** [July 9, 2024, 5:35pm UTC](https://community.mrtrix.org/t/issue-with-mtnormalise-non-positive-tissue-balance-factor-error/7648/2 "2024-07-09T17:35:00Z")

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> [@Nagore\_Castro](#):
>
> correctly

I’m also encountering the same issue, and would appreciate help

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**Author:** ![jdtournier](https://community.mrtrix.org/user_avatar/community.mrtrix.org/jdtournier/32/2594_2.png) [@jdtournier](https://community.mrtrix.org/u/jdtournier)\
**Post date:** [July 11, 2024, 7:27am UTC](https://community.mrtrix.org/t/issue-with-mtnormalise-non-positive-tissue-balance-factor-error/7648/3 "2024-07-11T07:27:24Z")

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Hi @polivaoren,

Have you checked your issue isn’t the same as [described in this post](https://community.mrtrix.org/t/mtnormalise-nan-values-as-balance-factors/4552/16)?

All the best,  
Donald