Manually extracted tract comparison

Good afternoon, I have two groups of subjects (10 control x 10 patients) and I would like to compare manually extracted U-fibers that connect the ventral pre and postcentral gyrus. I have some questions regarding this method:

  1. I have seen it in other papers, but I wonder whether this methodology is valid compared to TBSS or fixel-based analysis.

  2. I would like to know if any tract metric could be used in this comparison, including FA, MD, tract volume and number of streamlines.

  3. Finally, would it be necessary to draw ROIs with the same numbers of voxels and select the same number of streamlines for each subject in order to make this comparison possible?

Thank you

Thiago