# The following nodes do not have any streamlines assigned

**URL:** <https://community.mrtrix.org/t/the-following-nodes-do-not-have-any-streamlines-assigned/2712>\
**Category:** Uncategorized\
**Created:** [July 30, 2019, 1:09pm UTC](https://community.mrtrix.org/t/the-following-nodes-do-not-have-any-streamlines-assigned/2712 "2019-07-30T13:09:39Z")\
**Posts on this page:** 4\
**Page:** 2

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**Author:** ![Vaasudev](https://community.mrtrix.org/letter_avatar_proxy/v4/letter/v/4af34b/32.png) [@Vaasudev](https://community.mrtrix.org/u/Vaasudev)\
**Post date:** [July 31, 2019, 3:41pm UTC](https://community.mrtrix.org/t/the-following-nodes-do-not-have-any-streamlines-assigned/2712/21 "2019-07-31T15:41:44Z")

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Again i am facing the the problem

I tried applying this transform first to the labels

mrconvert –datatype uint32 hcpmmp1.mgz hcpmmp1.nii.gz

flirt -in hcpmmp1.nii.gz -ref norm\_2\_t1w.nii.gz -applyxfm -init norm\_2\_t1w.mat -out hcpmmp1\_labels.nii.gz

then i did

labelconvert hcpmmp1\_labels.nii.gz hcpmmp1\_original.txt hcpmmp1\_ordered.txt hcpmmp2\_parcels\_nocoreg.mif

now i am getting an error

labelconvert: Image “hcpmmp1\_labels.nii.gz” stored with floating-point type; need to check for non-integer or negative values  
labelconvert: [100%] uncompressing image “hcpmmp1\_labels.nii.gz”  
labelconvert: [100%] Verifying parcellation image  
labelconvert: [ERROR] Floating-point number detected in image “hcpmmp1\_labels.nii.gz”; label images should contain integers only.

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<div class="post-metadata">

**Author:** ![mblesac](https://community.mrtrix.org/user_avatar/community.mrtrix.org/mblesac/32/2210_2.png) [@mblesac](https://community.mrtrix.org/u/mblesac)\
**Post date:** [July 31, 2019, 3:45pm UTC](https://community.mrtrix.org/t/the-following-nodes-do-not-have-any-streamlines-assigned/2712/22 "2019-07-31T15:45:49Z")

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Hi,

When you apply a transformation to the labels with flirt, you should add the flag `-interp nearestneighbour` to the command.

Best regards,

Manuel

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<div class="post-metadata">

**Author:** ![Vaasudev](https://community.mrtrix.org/letter_avatar_proxy/v4/letter/v/4af34b/32.png) [@Vaasudev](https://community.mrtrix.org/u/Vaasudev)\
**Post date:** [July 31, 2019, 4:01pm UTC](https://community.mrtrix.org/t/the-following-nodes-do-not-have-any-streamlines-assigned/2712/23 "2019-07-31T16:01:11Z")

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Yes, i have done that but still i have a problem, for the label 120 the streamlines are not assigned

tck2connectome: [100%] Constructing connectome  
tck2connectome: [WARNING] The following nodes do not have any streamlines assigned:  
tck2connectome: [WARNING] 120  
tck2connectome: [WARNING] (This may indicate a poor registration)

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<div class="post-metadata">

**Author:** ![mblesac](https://community.mrtrix.org/user_avatar/community.mrtrix.org/mblesac/32/2210_2.png) [@mblesac](https://community.mrtrix.org/u/mblesac)\
**Post date:** [July 31, 2019, 4:11pm UTC](https://community.mrtrix.org/t/the-following-nodes-do-not-have-any-streamlines-assigned/2712/24 "2019-07-31T16:11:23Z")

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Hi,

This could be to several reasons:

- Small number of streamlines, 1million I think is very small number for this parcellation.
- It could be that this area is specially difficult to tract and you need to refine your tractography parameters
- Maybe remains unassigned, because all the area falls inside of the GM, and therefore no tracts are allowed to reach that part. I encountered this problem with some areas for some subjects (neonates).

First I would inspect the image, to see that are aligned and the node 120 is not all covered by GM, then I would create a tractography with 100million streamlines and see if the warning is still there. There is a paper discussing [some of these issues](https://www.sciencedirect.com/science/article/pii/S105381191930388X).

Best regards,

Manuel

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