7T Tractography and ROI

Hi

I have diffusion data at 7T + structural T1 and also hrT2 and a CISS (healthy volunteers)
I would like to perform probabilistic tractography of the CN VII/VIII complex.

  1. What would be the best way of registering the hrT2 to the diffusion data - would I just use flirt?
  2. What would the code look like for including my ROI - was going to use the IAM, facial colliculus and also the facial nucleus and draw these on the hrT2 and register that to the diffusion then do probabilistic tracts.

Just getting a bit muddled!