Please remember to load the reduced set of tracts (step 20)
Something else to consider/ be aware of is the -fmls_peak_value in step 12 and the -cutoff value in step 19.
The documentation states a value of 0.06 in both instances. However, this might be too low. In my case, 0.08 was a better choice as 0.06 gave too many spurious fixels/tracts in GM. 0.08 stroke a nice balance in reducing spurious fixels/tracts while retaining crossing fibres in WM.
As always, it is essential to evaluate the quality/validity of each of the processing steps.
See also discussion in post FBA pipeline - quality check for several steps
A nice overview article and associated code can be found here: GitHub - smeisler/Meisler_Reading_FBA: Code used in the Meisler and Gabrieli 2022 eLife paper on fixel based analyses of reading abilities.
Below are some screenshots. (1) FA study template, (2) whole brain tractogram 0.06, (3) tractogram 0.08
Cheers William
